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BIOASSAY DIRECTED ISOLATION AND CHARACTERIZATION OF BIOACTIVE COMPOUNDS FROM FRUIT PULP OF ANNONA MURICATA L
Thenmozhi A.*, Lenora L. M., Senthilkumar N. , Sri Durga Devi R. and Maleeka Begum S. F.
Annona muricata, a member of the Annonaceae family is a fruit tree with a long history of traditional and medicinal use. A. muricata, also known as Soursop, Graviola and Guanabana is an evergreen tree mostly distributed in tropical and subtropical regions of the world. Its fruits used to prepare ice creams, shakes, syrups and beverages. Information on this wild edible fruit is limited with reference to bioprospecting values. Hence, the study focused on the bioactive compounds present in the fruit pulp of A. muricata and their biological properties. Methanolic and Aqueous extracts of fruit pulp of A. muricata were subjected to the qualitative and quantitative phytochemical analysis. The Methanol and Aqueous extracts contains alkaloids (40% &8%), flavonoids (40% & 60%), terpenoids (50% & 50%), and saponins (10% & 80%) respectively. The GC-MS analysis of the methanolic extract revealed the presence of active compounds with anticancer activity which showed 65% inhibition of HeLa cell lines (MTT assay). The methanol and aqueous extracts have 74% and 65% of radical scavenging activity (DPPH assay). The extracts of fruit pulp showed mild antimicrobial activity against Bacteria, Bacillus Subtilis and Escherichia coli, and a fungus, Nigrospora oryzea. It contains nutritional factors such as carbohydrate, protein, fat, fiber, iron, vitamin A, vitamin C and sodium. Since A. muricata fruit contains good nutritional value, antioxidant and anticancer properties, it may be considered as a potential edible fruit for value addition.[Full Text Article]