Abstract
CONTROL OF THE GENOTOXICITY LEVEL OF CERTAIN MYCOTOXINS BY A FIBER OPTICAL SOS-TYPE BIOSENSOR
N. F. Starodub*, M. I. Fedelesh-Morenetz, M. V. Savchuk, O. P. Taran, O. I. Naboka and L. M. Shuliak
ABSTRACT
In addition to their well-known general toxicity, mycotoxins have also been found to often exert genotoxicity. In turn, not only the presence of the mycotoxins is required to be determined, but such DNA-damaging effects by these substances are also needed to be detected in routine monitoring of mycotoxin. The main purpose of this article is to demonstrate the utility of a proposed special fiber optical SOS-chromotest type biosensor, based on the SOS-lux assay in recombinant Escherichia coli cells, in rapid measurement of the genotoxicity level of mycotoxins. The optrode-based fiber optic biosensor and its main operating conditions for such express analysis have been worked out, moreover, its efficacy in the determination of the effects of certain investigated mycotoxins e.g.,T2 mycotoxin, patulin, aflatoxin B2, zearalenone and ochratoxin on the genetic apparatus applied and their activation of the operon of the reparative genes is reported. The genotoxicity of the mycotoxins studied fell in the same range, with genotoxicity order: T2 mycotoxin > aflatoxin B2 > patulin >> ochratoxin > zearalenone.
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