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World Journal of Engineering
Research and Technology

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Engineering Research and Technology

ISSN 2454-695X

Impact Factor : 5.924

ICV : 79.45

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Abstract

INVESTIGATION OF BACTERIAL DISEASES IN RAINBOW TROUT CAGES IN THE DOWN FIRAT BASIN

Mikail Özcan* and Fatma Burcu Kuzucu

ABSTRACT

The aim of the study was to examine bacterial diseases frequently seen in the fish in cage production and aquaculture farms operating in ?anl?urfa and Gaziantep provinces in Lower Euphrates Basin and to determine effective antibiotics against these disease agents. For this purpose, samples were taken from the liver, spleen, kidney and intestines of a total 140 Rainbow trout with the weight range of 10-700 gr located in different cage culture facilities and 3 hatcheries between October 2018 and May 2019. For isolation of bacterial disease agents, Tryptic Soy Agar (TSA), Nutrient Broth (NB), Brain-Heart Infusion Agar (BHIA), KF Streptococcus Agar, Yersinia Selective Agar and Tryptone Yeast Extract Salt Agar (TYES-A) were used. These culture media were incubated at 15-24oC for 24-72 hours in the incubator. Antibiogram sensitivity test on pure colonies was performed according to disc diffusion method. In addition to biochemical identification tests, Biolog System (The biolog GENIII micro plate) was applied to pure strains obtained from the samples taken from trout farms and their phenotypic properties were examined. As a conclusion, Lactococcus garviae, Aeromonas hydrophila-like DNA group 2, Acinetobacter schindler, Lactococcus lactis ss lactis, Leuconostoc mesenteroides ss cremoris and Morexella lacunata strains were isolated and identified according to the phenotypic and biochemical properties of the bacteria.

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