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World Journal of Engineering
Research and Technology

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Engineering Research and Technology

ISSN 2454-695X

Impact Factor : 5.924

ICV : 79.45

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Indexing

Abstract

BIOACTIVE PEPTIDES GENERATED FROM THE BY-PRODUCTS OF THE WHELK PROCESSING INDUSTRY

Daniela Varela*, Rosemary O’Hara and Adriana Cunha Neves

ABSTRACT

The worldwide fish and shellfish sector processes approximately one million ton of fish annually. However, only 43% of this is consumed, and important proteins are lost during product processing. The purpose of this research is to extract protein from industrial whelk processing byproducts and produce bioactive protein hydrolysates with marketable value. The whelk by-product extracted protein was hydrolyzed with Alcalase and ?- Chymotrypsin. The resulting peptides were tested for their antioxidant ferric reducing ability (FRAP) and Angiotensin-converting enzyme (ACE) inhibitory activity using spectrophotometric testing kits. Bacterial growth inhibition for e. coli and s.areus was also tested using the disk diffusion test. The ACE inhibition lowest IC50 value belongs to the acid extracted protein at 3,44?g/mL, non-processed protein was shown to have higher ACE inhibition than hydrolyzed protein, all samples had much higher IC50 values for ferric reducing ability when comparing to the IC50 for the DPPH activity performed in a previous study. Antibacterial activity was shown to be lower than other studies, although not powerful bacterial growth inhibitors in conjunction with the other bioactivities the peptides exhibit, if incorporated in a food product. These by-products have the potential to generate a new generation of multifunctional bioactive peptides that can be valuable to the industry as supplement/fortified meals and nutraceuticals.

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