Abstract
YOLK PROTEIN GENOME ORGANIZATION OF SILKWORM Bombyx mori (L) AND THEIR ASSOCIATION WITH EST BASED RFLP MARKER FOR SILKWORM CONSERVATION
Vishnu Priya S.*, Somasundaram P. and Kamble C. K.
ABSTRACT
Germplasm characterization is an important for conservation and utilization of genetic resources. DNA markers allow researchers to identify accessions at the taxonomic level, assess the relative diversity within and among species, and locate diverse accessions for breeding purposes. An investigation was carried out in the present study to identify the yolk protein gene related restriction fragment length polymorphism using expression sequence tag (EST) were used to differentiate high and low yolk yielding silkworm strains. Three restriction enzymes viz., Hinf I, EcoR I, Tag I were used in this study for digesting the EST-PCR product of yolk protein DNA of ten different silkworm races possessing contrasting silk for yolk yield. Among the selected races Kollegal Jawan , MY-1, Pure Mysore and Hosa Mysore were shown a polymorphic and total of six cluster group were observed on EST ?marker. The RFLP-PCR technique is flexible with regard to different environmental conditions as DNA is more stable. Using this study different silkworm strains will be characterized through restriction pattern and grouped together based on silk yield. This technique is rapid and inexpensive, enabling silkworm to be characterized in short periods.
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